Review

cer acyl cer standards (Avanti Polar)

Avanti Polar is a verified supplier

Avanti Polar manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Avanti Polar cer acyl cer standards

Greatly reduced but residual presence of PHS-containing CERs in the epidermis of Degs2 KO mice. Lipids were extracted from epidermis of postnatal day 0 WT (n = 3) and Degs2 KO (n = 3) mice, and CERs ( A and B ) and acyl-CERs ( C and D ) were measured via LC-MS/MS. Values presented are means + SD (∗∗ p < 0.01; Student’s t test) of the total quantities of <t>PHS-CERs,</t> <t>SPH-CERs,</t> and DHS-CERs ( A ); the quantity of each <t>PHS-CER</t> species containing the indicated FA moiety ( B ); the total quantities of acyl-PHS-CERs, acyl-SPH-CERs, and acyl-DHS-CERs ( C ); and the quantity of each acyl-PHS-CER species containing the indicated ω-hydroxy FA ( D ). CER, ceramide; DHS-CER, DHS containing CER; FA, fatty acid; PHS-CER, PHS-containing CER; SPH-CER, SPH-containing CER.

Cer Acyl Cer Standards, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cer+acyl+cer+standards/pmc10140171-245-15-48?v=Avanti+Polar
Average 94 stars, based on 19 article reviews

cer acyl cer standards - by Bioz Stars, 2026-08

94/100 stars

Images

1) Product Images from "Bifunctional DEGS2 has higher hydroxylase activity toward substrates with very-long-chain fatty acids in the production of phytosphingosine ceramides"

Article Title: Bifunctional DEGS2 has higher hydroxylase activity toward substrates with very-long-chain fatty acids in the production of phytosphingosine ceramides

Journal: The Journal of Biological Chemistry

doi: 10.1016/j.jbc.2023.104603

Figure Legend Snippet: Greatly reduced but residual presence of PHS-containing CERs in the epidermis of Degs2 KO mice. Lipids were extracted from epidermis of postnatal day 0 WT (n = 3) and Degs2 KO (n = 3) mice, and CERs ( A and B ) and acyl-CERs ( C and D ) were measured via LC-MS/MS. Values presented are means + SD (∗∗ p < 0.01; Student’s t test) of the total quantities of PHS-CERs, SPH-CERs, and DHS-CERs ( A ); the quantity of each PHS-CER species containing the indicated FA moiety ( B ); the total quantities of acyl-PHS-CERs, acyl-SPH-CERs, and acyl-DHS-CERs ( C ); and the quantity of each acyl-PHS-CER species containing the indicated ω-hydroxy FA ( D ). CER, ceramide; DHS-CER, DHS containing CER; FA, fatty acid; PHS-CER, PHS-containing CER; SPH-CER, SPH-containing CER.

Techniques Used: Liquid Chromatography with Mass Spectroscopy

Greatly reduced but residual presence of PHS-containing CERs in DEGS2 KO keratinocytes. A , DEGS2 KO keratinocytes were generated using the CRISPR/Cas9 system. The exon structure ( black , coding sequence; white , untranslated regions) of human DEGS2 and the nucleotide sequences of WT and DEGS2 KO keratinocytes (KO clones 1 and 2) around the guide RNA target sequences ( light blue ) and the protospacer-adjacent motif sequences ( magenta ) in exon 2 are shown. B – D , lipids were extracted from controls (controls 1 and 2) and DEGS2 KO keratinocytes (KO 1 and 2) differentiated for 14 days, and CERs and acyl-CERs were analyzed via LC-MS/MS. Values presented are means + SD (n = 3; ∗∗ p < 0.01; ∗ p < 0.05; Scheffé’s test) of the total quantities of PHS-CERs, SPH-CERs, DHS-CERs, acyl-PHS-CERs, acyl-SPH-CERs, and acyl-DHS-CERs ( B ); the quantity of each PHS-CER species containing the indicated FA moiety ( C ); and the quantity of each acyl-PHS-CER species containing the indicated ω-hydroxy FA ( D ). E , HEK 293T cells were transfected with pCE-puro 3× FLAG-1 (vector) or pCE-puro 3× FLAG-FA2H plasmid. After 24 h of transfection, lipids were extracted, and 2-hydroxy palmitic acid-containing SPH-CER (2-OH CER) and PHS-CERs were quantified via LC-MS/MS. Values presented are means + SD (n = 3; ∗∗ p < 0.01; ∗ p < 0.05; Student’s t test). CER, ceramide; DHS-CER, DHS containing CER; PHS-CER, PHS-containing CER; SPH-CER, SPH-containing CER.

Figure Legend Snippet: Greatly reduced but residual presence of PHS-containing CERs in DEGS2 KO keratinocytes. A , DEGS2 KO keratinocytes were generated using the CRISPR/Cas9 system. The exon structure ( black , coding sequence; white , untranslated regions) of human DEGS2 and the nucleotide sequences of WT and DEGS2 KO keratinocytes (KO clones 1 and 2) around the guide RNA target sequences ( light blue ) and the protospacer-adjacent motif sequences ( magenta ) in exon 2 are shown. B – D , lipids were extracted from controls (controls 1 and 2) and DEGS2 KO keratinocytes (KO 1 and 2) differentiated for 14 days, and CERs and acyl-CERs were analyzed via LC-MS/MS. Values presented are means + SD (n = 3; ∗∗ p < 0.01; ∗ p < 0.05; Scheffé’s test) of the total quantities of PHS-CERs, SPH-CERs, DHS-CERs, acyl-PHS-CERs, acyl-SPH-CERs, and acyl-DHS-CERs ( B ); the quantity of each PHS-CER species containing the indicated FA moiety ( C ); and the quantity of each acyl-PHS-CER species containing the indicated ω-hydroxy FA ( D ). E , HEK 293T cells were transfected with pCE-puro 3× FLAG-1 (vector) or pCE-puro 3× FLAG-FA2H plasmid. After 24 h of transfection, lipids were extracted, and 2-hydroxy palmitic acid-containing SPH-CER (2-OH CER) and PHS-CERs were quantified via LC-MS/MS. Values presented are means + SD (n = 3; ∗∗ p < 0.01; ∗ p < 0.05; Student’s t test). CER, ceramide; DHS-CER, DHS containing CER; PHS-CER, PHS-containing CER; SPH-CER, SPH-containing CER.

Techniques Used: Generated, CRISPR, Sequencing, Clone Assay, Liquid Chromatography with Mass Spectroscopy, Transfection, Plasmid Preparation

Preference for VLC substrates in the hydroxylation reaction by DEGS2. A and B , DEGS1 KO HAP1 cells were transfected with pEFh-3× FLAG-1 (vector), pEFh-3× FLAG-DEGS1, or pEFh-3× FLAG-DEGS2 plasmid. After 24 h, cells were labeled with 2 μM d 7 -DHS for 4 h. A , proteins were prepared and subjected to immunoblotting (IB) using anti-FLAG or anti-GAPDH (loading control) antibodies. B , lipids were extracted, and d 7 -labeled SPH-CERs, PHS-CERs, SPH-HexCERs, PHS-HexCERs, SPH-SMs, and PHS-SMs were quantified via LC-MS/MS. Values presented are means + SD of the respective lipids, with FA species color-coded (n = 3; ∗∗ p < 0.01; Dunnett’s test versus vector control). CER, ceramide; DHS-CER, DHS containing CER; FA, fatty acid; HexCER, monohexosylceramide; ND, not detected; PHS-CER, PHS-containing CER; SM, sphingomyelin; SPH-CER, SPH-containing CER.

Figure Legend Snippet: Preference for VLC substrates in the hydroxylation reaction by DEGS2. A and B , DEGS1 KO HAP1 cells were transfected with pEFh-3× FLAG-1 (vector), pEFh-3× FLAG-DEGS1, or pEFh-3× FLAG-DEGS2 plasmid. After 24 h, cells were labeled with 2 μM d 7 -DHS for 4 h. A , proteins were prepared and subjected to immunoblotting (IB) using anti-FLAG or anti-GAPDH (loading control) antibodies. B , lipids were extracted, and d 7 -labeled SPH-CERs, PHS-CERs, SPH-HexCERs, PHS-HexCERs, SPH-SMs, and PHS-SMs were quantified via LC-MS/MS. Values presented are means + SD of the respective lipids, with FA species color-coded (n = 3; ∗∗ p < 0.01; Dunnett’s test versus vector control). CER, ceramide; DHS-CER, DHS containing CER; FA, fatty acid; HexCER, monohexosylceramide; ND, not detected; PHS-CER, PHS-containing CER; SM, sphingomyelin; SPH-CER, SPH-containing CER.

Techniques Used: Transfection, Plasmid Preparation, Labeling, Western Blot, Liquid Chromatography with Mass Spectroscopy

Tissue distribution of t20:0 PHS-CERs and their production by DEGS2. A and B , lipids were extracted from six tissues (kidney, esophagus, anterior stomach, posterior stomach, small intestine, and large intestine) of 2-month-old WT mice (n = 3), and the quantities of SPH-CERs and PHS-CERs containing LCBs with chain lengths from d/t16 to d/t26 were quantified via LC-MS/MS. Values presented are means + SD of the total quantities of SPH-CERs and PHS-CERs ( A ; LCB chain lengths are color coded) and the quantities of t18:0- or t20:0-containing PHS-CERs ( B ; FA chain lengths are color-coded). C , lipids were extracted from the esophagus and anterior stomach of six-month-old WT (n = 3) and Degs2 KO (n = 3) mice, and the quantities of t18:0 and t20:0 PHS-CERs were examined via LC-MS/MS. Values presented are means + SD of the total quantities of PHS-CERs, with FA species color coded. D and E , esophagus and anterior stomach of 6-month-old WT and Degs2 KO mice were prepared and subjected to hematoxylin/eosin staining ( D ) and lucifer yellow assay ( E ). Scale bars, 50 μm ( D ) and 10 μm ( E ). F , lipids were extracted from the esophagus and anterior stomach of 6-month-old WT (n = 3) and Degs2 KO (n = 3) mice, and PHS-CERs, SPH-CERs containing 2-hydroxy FA (2-OH CERs), and SPH-CERs containing 3-hydroxy FA (3-OH CERs) were quantified via LC-MS/MS. Values presented are means + SD of the total quantities of PHS-CERs, 2-OH CERs, and 3-OH CERs. G and H , DEGS1 KO HAP1 cells were transfected with pCE-puro 3× FLAG-SPTLC1, pCE-puro 3× FLAG-SPTSSB, and pCE-puro HA-SPTLC3 plasmids together with pEFh-3× FLAG-1 (vector), pEFh-3× FLAG-DEGS1, or pEFh-3× FLAG-DEGS2 plasmid, and cultured for 24 h. G , proteins were prepared and subjected to immunoblotting (IB) with anti-FLAG, anti-HA, or anti-GAPDH (loading control) antibodies. H , lipids were extracted, and d20:1 SPH-CERs and t20:0 PHS-CERs were analyzed via LC-MS/MS. Values presented are means + SD of the total quantities of the respective lipids, with FA chain length color coded (n = 3; ∗∗ p < 0.01; Dunnett’s test versus vector control for d20:1 SPH-CERs and Student’s t test for t20:0 PHS-CERs). CER, ceramide; DHS-CER, DHS containing CER; FA, fatty acid; PHS-CER, PHS-containing CER; SPH-CER, SPH-containing CER.

Figure Legend Snippet: Tissue distribution of t20:0 PHS-CERs and their production by DEGS2. A and B , lipids were extracted from six tissues (kidney, esophagus, anterior stomach, posterior stomach, small intestine, and large intestine) of 2-month-old WT mice (n = 3), and the quantities of SPH-CERs and PHS-CERs containing LCBs with chain lengths from d/t16 to d/t26 were quantified via LC-MS/MS. Values presented are means + SD of the total quantities of SPH-CERs and PHS-CERs ( A ; LCB chain lengths are color coded) and the quantities of t18:0- or t20:0-containing PHS-CERs ( B ; FA chain lengths are color-coded). C , lipids were extracted from the esophagus and anterior stomach of six-month-old WT (n = 3) and Degs2 KO (n = 3) mice, and the quantities of t18:0 and t20:0 PHS-CERs were examined via LC-MS/MS. Values presented are means + SD of the total quantities of PHS-CERs, with FA species color coded. D and E , esophagus and anterior stomach of 6-month-old WT and Degs2 KO mice were prepared and subjected to hematoxylin/eosin staining ( D ) and lucifer yellow assay ( E ). Scale bars, 50 μm ( D ) and 10 μm ( E ). F , lipids were extracted from the esophagus and anterior stomach of 6-month-old WT (n = 3) and Degs2 KO (n = 3) mice, and PHS-CERs, SPH-CERs containing 2-hydroxy FA (2-OH CERs), and SPH-CERs containing 3-hydroxy FA (3-OH CERs) were quantified via LC-MS/MS. Values presented are means + SD of the total quantities of PHS-CERs, 2-OH CERs, and 3-OH CERs. G and H , DEGS1 KO HAP1 cells were transfected with pCE-puro 3× FLAG-SPTLC1, pCE-puro 3× FLAG-SPTSSB, and pCE-puro HA-SPTLC3 plasmids together with pEFh-3× FLAG-1 (vector), pEFh-3× FLAG-DEGS1, or pEFh-3× FLAG-DEGS2 plasmid, and cultured for 24 h. G , proteins were prepared and subjected to immunoblotting (IB) with anti-FLAG, anti-HA, or anti-GAPDH (loading control) antibodies. H , lipids were extracted, and d20:1 SPH-CERs and t20:0 PHS-CERs were analyzed via LC-MS/MS. Values presented are means + SD of the total quantities of the respective lipids, with FA chain length color coded (n = 3; ∗∗ p < 0.01; Dunnett’s test versus vector control for d20:1 SPH-CERs and Student’s t test for t20:0 PHS-CERs). CER, ceramide; DHS-CER, DHS containing CER; FA, fatty acid; PHS-CER, PHS-containing CER; SPH-CER, SPH-containing CER.

Techniques Used: Liquid Chromatography with Mass Spectroscopy, Staining, Transfection, Plasmid Preparation, Cell Culture, Western Blot

Image Search Results

Journal: The Journal of Biological Chemistry

Article Title: Bifunctional DEGS2 has higher hydroxylase activity toward substrates with very-long-chain fatty acids in the production of phytosphingosine ceramides

doi: 10.1016/j.jbc.2023.104603

Figure Lengend Snippet: Greatly reduced but residual presence of PHS-containing CERs in the epidermis of Degs2 KO mice. Lipids were extracted from epidermis of postnatal day 0 WT (n = 3) and Degs2 KO (n = 3) mice, and CERs ( A and B ) and acyl-CERs ( C and D ) were measured via LC-MS/MS. Values presented are means + SD (∗∗ p < 0.01; Student’s t test) of the total quantities of PHS-CERs, SPH-CERs, and DHS-CERs ( A ); the quantity of each PHS-CER species containing the indicated FA moiety ( B ); the total quantities of acyl-PHS-CERs, acyl-SPH-CERs, and acyl-DHS-CERs ( C ); and the quantity of each acyl-PHS-CER species containing the indicated ω-hydroxy FA ( D ). CER, ceramide; DHS-CER, DHS containing CER; FA, fatty acid; PHS-CER, PHS-containing CER; SPH-CER, SPH-containing CER.

Article Snippet: To these samples, we added 375 μl of chloroform/methanol (1:2, v/v) and d 9 -labeled CER/acyl-CER standards ( d 9 -SPH-CER, 10 pmol; d 9 -DHS-CER, 5 pmol; d 9 -PHS-CER, 2.5 pmol; N -(26-oleoyloxy( d 9 ) hexacosanoyl) D- erythro -SPH [ d 9 -acyl-SPH-CER], 10 pmol [Avanti Polar Lipids]) and mixed vigorously.

Techniques: Liquid Chromatography with Mass Spectroscopy

Journal: The Journal of Biological Chemistry

Article Title: Bifunctional DEGS2 has higher hydroxylase activity toward substrates with very-long-chain fatty acids in the production of phytosphingosine ceramides

doi: 10.1016/j.jbc.2023.104603

Figure Lengend Snippet: Greatly reduced but residual presence of PHS-containing CERs in DEGS2 KO keratinocytes. A , DEGS2 KO keratinocytes were generated using the CRISPR/Cas9 system. The exon structure ( black , coding sequence; white , untranslated regions) of human DEGS2 and the nucleotide sequences of WT and DEGS2 KO keratinocytes (KO clones 1 and 2) around the guide RNA target sequences ( light blue ) and the protospacer-adjacent motif sequences ( magenta ) in exon 2 are shown. B – D , lipids were extracted from controls (controls 1 and 2) and DEGS2 KO keratinocytes (KO 1 and 2) differentiated for 14 days, and CERs and acyl-CERs were analyzed via LC-MS/MS. Values presented are means + SD (n = 3; ∗∗ p < 0.01; ∗ p < 0.05; Scheffé’s test) of the total quantities of PHS-CERs, SPH-CERs, DHS-CERs, acyl-PHS-CERs, acyl-SPH-CERs, and acyl-DHS-CERs ( B ); the quantity of each PHS-CER species containing the indicated FA moiety ( C ); and the quantity of each acyl-PHS-CER species containing the indicated ω-hydroxy FA ( D ). E , HEK 293T cells were transfected with pCE-puro 3× FLAG-1 (vector) or pCE-puro 3× FLAG-FA2H plasmid. After 24 h of transfection, lipids were extracted, and 2-hydroxy palmitic acid-containing SPH-CER (2-OH CER) and PHS-CERs were quantified via LC-MS/MS. Values presented are means + SD (n = 3; ∗∗ p < 0.01; ∗ p < 0.05; Student’s t test). CER, ceramide; DHS-CER, DHS containing CER; PHS-CER, PHS-containing CER; SPH-CER, SPH-containing CER.

Techniques: Generated, CRISPR, Sequencing, Clone Assay, Liquid Chromatography with Mass Spectroscopy, Transfection, Plasmid Preparation

Journal: The Journal of Biological Chemistry

Article Title: Bifunctional DEGS2 has higher hydroxylase activity toward substrates with very-long-chain fatty acids in the production of phytosphingosine ceramides

doi: 10.1016/j.jbc.2023.104603

Figure Lengend Snippet: Preference for VLC substrates in the hydroxylation reaction by DEGS2. A and B , DEGS1 KO HAP1 cells were transfected with pEFh-3× FLAG-1 (vector), pEFh-3× FLAG-DEGS1, or pEFh-3× FLAG-DEGS2 plasmid. After 24 h, cells were labeled with 2 μM d 7 -DHS for 4 h. A , proteins were prepared and subjected to immunoblotting (IB) using anti-FLAG or anti-GAPDH (loading control) antibodies. B , lipids were extracted, and d 7 -labeled SPH-CERs, PHS-CERs, SPH-HexCERs, PHS-HexCERs, SPH-SMs, and PHS-SMs were quantified via LC-MS/MS. Values presented are means + SD of the respective lipids, with FA species color-coded (n = 3; ∗∗ p < 0.01; Dunnett’s test versus vector control). CER, ceramide; DHS-CER, DHS containing CER; FA, fatty acid; HexCER, monohexosylceramide; ND, not detected; PHS-CER, PHS-containing CER; SM, sphingomyelin; SPH-CER, SPH-containing CER.

Techniques: Transfection, Plasmid Preparation, Labeling, Western Blot, Liquid Chromatography with Mass Spectroscopy

Journal: The Journal of Biological Chemistry

Article Title: Bifunctional DEGS2 has higher hydroxylase activity toward substrates with very-long-chain fatty acids in the production of phytosphingosine ceramides

doi: 10.1016/j.jbc.2023.104603

Figure Lengend Snippet: Tissue distribution of t20:0 PHS-CERs and their production by DEGS2. A and B , lipids were extracted from six tissues (kidney, esophagus, anterior stomach, posterior stomach, small intestine, and large intestine) of 2-month-old WT mice (n = 3), and the quantities of SPH-CERs and PHS-CERs containing LCBs with chain lengths from d/t16 to d/t26 were quantified via LC-MS/MS. Values presented are means + SD of the total quantities of SPH-CERs and PHS-CERs ( A ; LCB chain lengths are color coded) and the quantities of t18:0- or t20:0-containing PHS-CERs ( B ; FA chain lengths are color-coded). C , lipids were extracted from the esophagus and anterior stomach of six-month-old WT (n = 3) and Degs2 KO (n = 3) mice, and the quantities of t18:0 and t20:0 PHS-CERs were examined via LC-MS/MS. Values presented are means + SD of the total quantities of PHS-CERs, with FA species color coded. D and E , esophagus and anterior stomach of 6-month-old WT and Degs2 KO mice were prepared and subjected to hematoxylin/eosin staining ( D ) and lucifer yellow assay ( E ). Scale bars, 50 μm ( D ) and 10 μm ( E ). F , lipids were extracted from the esophagus and anterior stomach of 6-month-old WT (n = 3) and Degs2 KO (n = 3) mice, and PHS-CERs, SPH-CERs containing 2-hydroxy FA (2-OH CERs), and SPH-CERs containing 3-hydroxy FA (3-OH CERs) were quantified via LC-MS/MS. Values presented are means + SD of the total quantities of PHS-CERs, 2-OH CERs, and 3-OH CERs. G and H , DEGS1 KO HAP1 cells were transfected with pCE-puro 3× FLAG-SPTLC1, pCE-puro 3× FLAG-SPTSSB, and pCE-puro HA-SPTLC3 plasmids together with pEFh-3× FLAG-1 (vector), pEFh-3× FLAG-DEGS1, or pEFh-3× FLAG-DEGS2 plasmid, and cultured for 24 h. G , proteins were prepared and subjected to immunoblotting (IB) with anti-FLAG, anti-HA, or anti-GAPDH (loading control) antibodies. H , lipids were extracted, and d20:1 SPH-CERs and t20:0 PHS-CERs were analyzed via LC-MS/MS. Values presented are means + SD of the total quantities of the respective lipids, with FA chain length color coded (n = 3; ∗∗ p < 0.01; Dunnett’s test versus vector control for d20:1 SPH-CERs and Student’s t test for t20:0 PHS-CERs). CER, ceramide; DHS-CER, DHS containing CER; FA, fatty acid; PHS-CER, PHS-containing CER; SPH-CER, SPH-containing CER.

Techniques: Liquid Chromatography with Mass Spectroscopy, Staining, Transfection, Plasmid Preparation, Cell Culture, Western Blot

Review

Structured Review

Images

1) Product Images from "Bifunctional DEGS2 has higher hydroxylase activity toward substrates with very-long-chain fatty acids in the production of phytosphingosine ceramides"

Similar Products

Image Search Results